Pengaruh Pemberian Ekstrak Rosella (Hibiscus Sabdariffa Linn) Terhadap Jumlah Dan Kecepatan Spermatozoa, Berat Testis Tikus Jantan Strain Wistar Yang Terpapar Karbon Tetraklorida (CCl4)

TITIN IFAYANTI, Fauzan Abdullah

Abstract


Abstrak—Pemberian karbontetraklorida (CCl4) pada tikus jantan strain wistar menyebabkan peningkatan radikal bebas. Karbontetraklorida berfungsi sebagai radikal bebas sehingga terjadi akomodasi radikal bebas yaitu CCl3 yang merupakan zat reaktif. CCl3 bereaksi dengan oksigen menghasilkan CCl3O2 bersifat sangat reaktif meyebabkan peningkatan Reactive Oxygen Species (ROS). Radikal bebas yang berlebihan mengganggu proses

 spermatogenesis, kerusakan membran spermatozoa akibat terbentuknya lipid peroksida pada membran akan menurunkan jumlah, kecepatan spermatozoa dan berat testis, sehingga diberikan antioksidan ekstrak rosella. 

Desain penelitian merupakan penelitian eksperimental laboratoris menggunakan rancangan penelitian post test only control group yaitu digunakan untuk mengukur pengaruh perlakuan pada kelompok eksperimen, membandingkan perlakuan dengan kelompok kontrol. Hasil penelitian jumlah spermatozoa tikus jantan yang terpapar karbontetraklorida (CCl4) terdapat perbedaan bermakna antara kelompok kontrol negatif dengan kelompok kontrol positif serta pada kelompok perlakuan I dan perlakuan II karena nilai p≤0,05. Pada kecepatan spermatozoa menunjukkan perbedaan bermakna antara kelompok kontrol negatif dengan kelompok kontrol positif, kelompok perlakuan I dan perlakuan II karena nilai p≤0,05. Pada berat testis tidak menunjukkan pengaruh yang bermakna, dimana p>0,05.

Kesimpulan penelitian ini, pemberian ekstrak rosella dosis 250 dan 500 mg/kgbb memberi pengaruh secara signifikan terhadap peningkatan jumlah dan kecepatan spermatozoa pada tikus yang terpapar CCl4, sedangkan pada berat testis tidak terdapat pengaruh yang signifikan.

 

Kata kunci : Ekstrak rosella, Karbontetraklorida (CCl4), Jumlah Spermatozoa, Kecepatan     Spermatozoa

 

Abstract— Administration of Carbon Tetrachloride to male Wistar Rats cause the increase of free radicals. Carbon Tetrachloride served as free radicals which resulted in free radicals accomodation, CCl3, which is reactive substance, occured. CCl3 reactive oxygen and produced  CCl3 O2 , which was highly reactive, and caused the increase of Reactive Oxygen Species (ROS). Excessive free radicals would disturb the process of spermatogenesis. The damage to spermatozoa membrane which was caused by the formation of lipid peroxides at membrane would decrease the number and the speed of spermatozoa, and the weight of the testicle, so that antioxidant of Rosella extract was given.

Teh design of the research was laboratory experimental recearch using post test only control group research design which was used to measure the effect to the treatment in the experimental group by comparing it to treatment of the control group.

From the research of the number of spermatozoa of the male rats exposed to Carbon Tetrachloride (CCl4), it was found out that there was a significant difference between the number of the spermatozoa of negative control group and the positive control group, and at treatment group I and treatment group II beacuse the p value was ≤ 0,05. It was also found out that there was a significant difference of the spermatozoa speed between the negative control group and the positive control group, and at treatment group I and treatment group II because the p value was ≤ 0,05. On the other hand, there was no significant difference of the testicle weight in which the p value > 0,05.

 The conclusion of the research was that Rosella extract administration with 250 and 500 mg/kgbw doses gave significant influence to the icrease of the number and the speed of spermatozoa of rat exposed to CCl4, whereas there was no significant influence on the testicle weight.

 

Key word: Rosella Extract, Carbon Tetrachloride, The Number of Spermatozoa, To Speed of Spermatozoa


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References


DAFTAR PUSTAKA

Amin A, Hamza, A. 2005. Hepatoprotective effects of Hisbiscus, Rosmarinus and Salvia on azathioprine-induced toxicity in rats. Life Sci. 77 (3): 266-278.

Agarwal, Ashok, Makker, Kartikeya, Sharma, Rakesh. 2007. Clinical Relevance of Oxidative Stress in Male Factor Infertility: an update. http://ccf.org/reproductiveresearchcenter/docs/agradoc261.pdf. Diakses bulan Agustus 2016.

Ali, B.H, N.A. Wabel< & G. Blunden. 2005. Phytochemical, pharmacological, And toxicological aspects of Hebiscus sabdariffa L. : A review.

Phytotherapy Researh 19 : 369-375

Chevion S, Moran D.S, Heled Y, Shani Y, Regrev G, Abbou B, et all. 2003. Plasma Antioxidant Status and Cell Injury After Severe Physical Exercise. Proc Nati Acad Sci. 100(9): 5119-5123.

Dahiru D, Obi O.J, Umaru, H. 2003. Effect of Hibiscus sabdariffa calyx extract on carbon tetrachloride induced liver damage. Biokemistri 15(1): 27-33.

Feter J, Csomos G and Vereckel A. 1992. Role of fret radical reaction in liver disease. Free radical and the liver, Csomos G and Feter J (eds). Springer Verlag. Berlin.

Hinting A. 2008. Study protokol penatalaksanaan dan efektivitas pengobatan infertilitas pria. http://digilib.litbang.depkes.go:id/go.pid=skpkbppk-gdl-res-2000-aucky-989inferlitit&node=124&start=836. Diakses bulan Agustus 2016.

Heim, K.E., A.R. Tagliaferro, & D.J. Bobilya. 2001. Flavonoid antioxidants:Chemistry, metabolism and structure-activity relationships. Journal ofNutritional Biochemistry 13: 572--584

Hirunpanich, V., A. Utaipat, N.P. Morales, N. Bunyapraphatsara, H.Sato, A.Herunsale,& C. Suthisisang .2005 Antioxidant effects of aquous extracts From dried calyx of Hibiscus sabdariffa Linn. (roselle) in vitri using rat Low-density lipoprotein (LDL). Biology Pharmacy Bulettin 28(3) : 481- 484

Nijveldt, R.J., E.V. Nood, D.E.C van Horrn, P.G. Boelens, K.V.Norren, & P.A.M. van Leewen. 2001. Flavonoids: A review of probable mechanisms of action and potential applications. American Journal Clinical Nutrition 74: 418-425

Oteiza, P.I., A.G. Erlejman, S.V. Verstraeten, C.L. Keen, & C.G. Fraga. 2005 Flavonoid-membrane interaction: A protective role of flavonoidscat the Membrane surface? Clinical and Developmental Immunology 12(1): 19—25

Pangkahila W. 2007. Memperlambat Penuaan Meningkatkan Kualitas Hidup. Anti-Aging Medicine. Cetakan ke-1. Jakarta. Penerbit Buku Kompas. hal:8-11.

Saleh, Ramadan A, Agarwal, Ashok. 2002. Oxidative Stress and Male Infertility: From Research Beach to Clinical Practice. J. Androl. 23(6):737-752. www.clevelandclinic.org/ReproductiveResearchCenter/docs/agradocs110.pdf. Diakses pada bulan Agustus 2016.

Siti, Untari Subekti. 2009. Pengaruh vitamin E terhadap Proses Spermatogenesis Mancit Jantan Strain B/C yang diberi Paparan Asap Rokok

Wang, H., G. Cao, & R.L. Prior. 1997.Oxygen radical absorbing capacity of Anthocyanins. Journal Agricultural Food Chemistry 45: 304--309

World Health Organization. 2005. Infertility.

http://www.who.int/reproductivehealth/infertility/index.htm. Diakses pada bulan Agustus 2016.

WHO. 2002. Selenium. Human Vitamin and Mineral. FHO/WHO. Roma


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